The European flounder (Platichthys flesus) TP53 functions as a temperature-sensitive transcription factor which inhibits cell growth in yeast.
Identifieur interne : 000D57 ( France/Analysis ); précédent : 000D56; suivant : 000D58The European flounder (Platichthys flesus) TP53 functions as a temperature-sensitive transcription factor which inhibits cell growth in yeast.
Auteurs : J. Cachot [France] ; J M Flaman ; T. Frébourg ; F. LeboulengerSource :
- Gene [ 0378-1119 ] ; 2004.
English descriptors
- KwdEn :
- Animals, Cell Division (genetics), Cell Division (physiology), Flounder (genetics), Genetic Vectors (genetics), Humans, Mutation, Missense, Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Temperature, Time Factors, Transcription Factors (genetics), Transcription Factors (physiology), Transcription, Genetic, Tumor Suppressor Protein p53 (genetics), Tumor Suppressor Protein p53 (physiology).
- MESH :
- chemical , genetics : Transcription Factors, Tumor Suppressor Protein p53.
- genetics : Cell Division, Flounder, Genetic Vectors, Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- physiology : Cell Division, Transcription Factors, Tumor Suppressor Protein p53.
- Animals, Humans, Mutation, Missense, Temperature, Time Factors, Transcription, Genetic.
Abstract
Numerous studies focus on biological roles of the TP53 tumor suppressor gene in mammals but little is known about the actual function of TP53 in lower vertebrates. In this study, we used an in vivo functional assay in yeast to address the transactivation capacity of the flounder TP53 protein. We showed that the flounder TP53 acts as a sequence-specific transcription factor which is able to transactivate various human promoters containing a p53-responsive element (RE). This transcriptional activity was completely abrogated in the Val147Glu TP53 mutant previously identified in two flounder hepatic hyperplasia. In addition, we showed that the wild-type (wt) flounder TP53 but not the Val147Glu mutant inhibits cell growth when expressed in yeast. We finally reported that transcription regulation and growth inhibition by the wild-type flounder TP53 is temperature-dependent. The flounder TP53 optimal temperature appeared lower than those reported for the Xenopus and human homologues.
PubMed: 14693375
Affiliations:
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pubmed:14693375Le document en format XML
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Lebon, Le Havre Cedex</wicri:regionArea><wicri:noRegion>Le Havre Cedex</wicri:noRegion><wicri:noRegion>Le Havre Cedex</wicri:noRegion></affiliation></author><author><name sortKey="Flaman, J M" sort="Flaman, J M" uniqKey="Flaman J" first="J M" last="Flaman">J M Flaman</name></author><author><name sortKey="Frebourg, T" sort="Frebourg, T" uniqKey="Frebourg T" first="T" last="Frébourg">T. Frébourg</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. 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Cachot</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Ecotoxicology, University of Le Havre, 25 rue P. Lebon, Le Havre Cedex, France. Jerome.Cachot@univ.lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology, University of Le Havre, 25 rue P. Lebon, Le Havre Cedex</wicri:regionArea><wicri:noRegion>Le Havre Cedex</wicri:noRegion><wicri:noRegion>Le Havre Cedex</wicri:noRegion></affiliation></author><author><name sortKey="Flaman, J M" sort="Flaman, J M" uniqKey="Flaman J" first="J M" last="Flaman">J M Flaman</name></author><author><name sortKey="Frebourg, T" sort="Frebourg, T" uniqKey="Frebourg T" first="T" last="Frébourg">T. Frébourg</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Division (genetics)</term><term>Cell Division (physiology)</term><term>Flounder (genetics)</term><term>Genetic Vectors (genetics)</term><term>Humans</term><term>Mutation, Missense</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Temperature</term><term>Time Factors</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (physiology)</term><term>Transcription, Genetic</term><term>Tumor Suppressor Protein p53 (genetics)</term><term>Tumor Suppressor Protein p53 (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transcription Factors</term><term>Tumor Suppressor Protein p53</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Division</term><term>Flounder</term><term>Genetic Vectors</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Division</term><term>Transcription Factors</term><term>Tumor Suppressor Protein p53</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Mutation, Missense</term><term>Temperature</term><term>Time Factors</term><term>Transcription, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Numerous studies focus on biological roles of the TP53 tumor suppressor gene in mammals but little is known about the actual function of TP53 in lower vertebrates. In this study, we used an in vivo functional assay in yeast to address the transactivation capacity of the flounder TP53 protein. We showed that the flounder TP53 acts as a sequence-specific transcription factor which is able to transactivate various human promoters containing a p53-responsive element (RE). This transcriptional activity was completely abrogated in the Val147Glu TP53 mutant previously identified in two flounder hepatic hyperplasia. In addition, we showed that the wild-type (wt) flounder TP53 but not the Val147Glu mutant inhibits cell growth when expressed in yeast. We finally reported that transcription regulation and growth inhibition by the wild-type flounder TP53 is temperature-dependent. The flounder TP53 optimal temperature appeared lower than those reported for the Xenopus and human homologues.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Flaman, J M" sort="Flaman, J M" uniqKey="Flaman J" first="J M" last="Flaman">J M Flaman</name><name sortKey="Frebourg, T" sort="Frebourg, T" uniqKey="Frebourg T" first="T" last="Frébourg">T. Frébourg</name><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></noCountry><country name="France"><noRegion><name sortKey="Cachot, J" sort="Cachot, J" uniqKey="Cachot J" first="J" last="Cachot">J. Cachot</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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